Speaker
Description
Marcin Libera, PhD, Eng.
The lecture focuses on modern cryo-electron microscopy (cryo-EM) techniques, with particular emphasis on sample preparation methods. It discusses key freezing strategies such as plunge freezing, high-pressure freezing, and slam freezing, along with approaches to fixation and rapid flash freezing of biological material. The lecture explains the importance of vitrification in protecting samples from electron beam damage and outlines the principles of low-dose microscopy. It also introduces the fundamentals of cryo-TEM tomography and the process of 3D reconstruction from tilt-series images. Overall, the session provides a comprehensive overview of techniques that enable high-resolution structural analysis while preserving the native state of specimens.